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KMID : 0644320000060010099
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Journal of Korean Oriental Oncology
2000 Volume.6 No. 1 p.99 ~ p.111
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Studies on Expression of Cell Cycle Related Genes in HL-60 Cells Undergoing Apoptosis by the Methanol Extract of Hedyotis diffusa
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Han Se-Hee
Lee Jong-Bum
Moon Goo
Moon Seok-Jae
Won Jin-Hee
Park Rae-Gil
Lee Jong-Deuk
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Abstract
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Objecives: Hedyotis diffusa is used to treat cancer in traditional Korea Medicine. So this study was carried out to examine the expression of cell cycle related genes in HL-60 cells undergoing apoptosis by the methanol extract of Hedyotis diffusa.
Methods:
1. HL-60 cells were treated with various concentrations (from 200 to 50㎍/ml)of metnanol extract and H20 extract (200 ㎍/ml) of hedyotis diffusa. After 48 h later, the cells were tested for viability by MTT assay.
2. The HL-60 cells were treated with 200 ㎍/ml of methanol extract for the indicated periods. The whole cell lysates were prepared and analyzed by westem blotting using anti-p53 antibody.
3. The nuclear extract were prepared and analyed by western blotting using anti-p21 antibody, anti-p27 antibody, anti-cyclen A antibody, anti-cylin E antibody and anti-CDK2 antinbody.
Results:
1. The methanol extract of Hedyotis diffusa induced the death of HL-60 cells in a dose dependent manner.
2. The methanol extract of Hedyotis diffusa makedly decreased the level of p21/Cipl and cyclin A in a time dependent manner.
3. The methanol extract of Hedyotis diffusa markedly increased tje ;eve; pf p27/Kip and cyclin E in a time dependent mammer.
4.The methanol extract of Hedyotis diffusa markedly did not affect the level of CDK2.
Conclusions: These results provide evidence that expression of cell cycle related genes in HL-6- cells undergoing apoptosis by the methanol extract of Hedyotis diffusa mainly results from decreased level of p21/cipl and increased level of p27/Kipl of the cell cycle related genes.
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